Journal: Biomaterials science

Article Title: Toward Reducing Biomaterials Antigenic Potential: A Miniaturized Fc-Binding Domain for Local Deposition of Antibodies

doi: 10.1039/c8bm01220b

Figure Lengend Snippet: Simulation of antibody in footpad injection depot. (a) One-compartment model diagramed using the Matlab Simbiology module (2017a) with the simulations governed by the equations described in Methods and Materials. Since it was assumed that no other anatomical structure or physiological mechanism was involved in the clearance, the depot is the single compartment in which the gel was injected, with “Ab_Z15EAK” representing IgG800 complexed with Z15_EAK/EAK coacervate, “Ab” representing dissociated IgG800, and “Z15EAK” representing Z15_EAK and EAK (which were assumed to remain always in the depot); reaction_1 represents reversible binding (kon and koff) between antibody and Z15, and reaction_2 represents elimination of antibody from the depot (kel); (b) Fitting of in vivo decay data in Fig. 6 to first-order elimination model to obtain kel; (d) Simulated profiles of the complex, Z15_EAK/EAK composite, and free antibody using Kd determined in ITC and the microplate assay. AUC was calculated using a trapezoidal numerical integration function in Matlab.

Article Snippet: Calculations of the relative exposures (AUC) indicate 0.27 fraction of the antibody injected existed as unbound during the 28-day period. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 7: caption a7 Simulation of antibody in footpad injection depot. (a) One-compartment model diagramed using the Matlab Simbiology module (2017a) with the simulations governed by the equations described in Methods and Materials.

Techniques: Injection, Binding Assay, In Vivo

Journal: Biomaterials science

Article Title: Toward Reducing Biomaterials Antigenic Potential: A Miniaturized Fc-Binding Domain for Local Deposition of Antibodies

doi: 10.1039/c8bm01220b

Figure Lengend Snippet: Simulation of antibody in footpad injection depot. (a) One-compartment model diagramed using the Matlab Simbiology module (2017a) with the simulations governed by the equations described in Methods and Materials. Since it was assumed that no other anatomical structure or physiological mechanism was involved in the clearance, the depot is the single compartment in which the gel was injected, with “Ab_Z15EAK” representing IgG800 complexed with Z15_EAK/EAK coacervate, “Ab” representing dissociated IgG800, and “Z15EAK” representing Z15_EAK and EAK (which were assumed to remain always in the depot); reaction_1 represents reversible binding (kon and koff) between antibody and Z15, and reaction_2 represents elimination of antibody from the depot (kel); (b) Fitting of in vivo decay data in Fig. 6 to first-order elimination model to obtain kel; (d) Simulated profiles of the complex, Z15_EAK/EAK composite, and free antibody using Kd determined in ITC and the microplate assay. AUC was calculated using a trapezoidal numerical integration function in Matlab.

Article Snippet: Matlab 2017b with a Simbiology module was utilized to simulate concentration of unbound IgG upon footpad injection with Z15_EAK/EAK.

Techniques: Injection, Binding Assay, In Vivo